In detail
HPLC passes a prepared sample through a column that separates compounds by their interaction with the stationary and mobile phases. A detector — commonly UV/DAD or mass spectrometry — measures what elutes. Because the compounds are separated first, HPLC can report a single molecule such as piperine or trans-resveratrol, or a defined set of compounds such as ginkgolides.
The result depends on the method: column, mobile phase, gradient, wavelength, reference standard, and calculation basis. A certificate that states 'HPLC' without the method is better than no method, but still not fully comparable. For high-value botanicals, ask for a chromatogram as well as the percentage.
Why it matters to a buyer
- It supports specific label claims for individual compounds or defined marker families.
- It is more specific than UV-VIS, which often measures a family against one standard.
- Method differences explain why two suppliers can quote different numbers for the same grade.
What to ask a supplier
Which column, mobile phase, and reference standard were used?
Is the result reported as-is or on a dry basis?
Can you provide a representative chromatogram?
References and further reading
External links are provided for orientation. Requirements differ by market, product, and dose; confirm with your regulatory advisor.
Cite this page
Rainwood Botanical. "High-performance liquid chromatography." Botanical extract glossary, October 2026. https://ingredients.rainwoodbio.com/resources/glossary/hplc/